Dietary replacement of soybean meal with heat-treated grain soybean in diets of feedlot-finished beef cattle: impacts on intake, digestibility, and ruminal parameters.

The objective of this study was to assess the impact of increasing levels of heat-treated soybean in the diet of crossbred cattle during the finishing phase on nutrient intake and digestibility, ruminal parameters, digesta passage rate, nitrogen balance, and microbial protein synthesis. Five steers, crossbred 7/8 Jersey x Zebu, fitted with rumen cannulas and with an average weight of 350 ± 50 kg, were utilized. The experimental treatments consisted of 0, 7, 14, 21, and 28% inclusion of heat-treated soybean in the total diet dry matter. The animals were randomly allocated in a 5 × 5 Latin square design. Evaluation of the animals took place over five experimental periods, each lasting 20 days. During each experimental period, the first 15 days were allocated for animal adaptation to the experimental diets, followed by five days of data collection. No significant differences were observed among the diets in terms of dry matter intake (average of 6.57 kg day-1; P = 0.615) and organic matter intake (average of 6.23 kg day-1; P = 0.832). However, heat-treated soybean had a significant impact on the digestibility of dry matter (P = 0.02), organic matter (P = 0.01), crude protein (P < 0.01), and neutral detergent fiber (P < 0.01). There was no observed change on microbial protein synthesis (average of 409.6 g day-1) in animals with the inclusion of heat-treated soybean in the diets. With each 1% inclusion of heat-treated soybean in the cattle diet, there was an increase of 0.00754 units in ruminal pH values and a reduction of 0.75839 mg dL-1 in ruminal ammoniacal nitrogen values. This study suggests that heat-treated soybean can be included in up to 15% of the dry matter in diets for finishing feedlot cattle.

Effect of increasing doses of chitosan to grazing beef steers on the relative population and transcript abundance of Archaea and cellulolytic and amylolytic bacterias.

This paper aims to investigate the influence of increasing chitosan doses on the relative proportion and abundance of cellulotytic, amylolytic bacteria, and Archaea transcripts for grazing cattle. Five rumen cannulated crossbread steers [3.6 months and 300 ± 25 kg body LW (live weight), mean ± standard deviation] were used in a 5 × 5 latin square design, randomly assigned to treatment sequence containing chitosan added to 0, 400, 800, 1200, or 1600 mg/kg concentrate. There was the effect of chitosan on the population of Fibrobacter succinogenes, Ruminococcus albus, and Archaea. The lowest population of these bacteria of 576.60 mg/kg DM (dry matter), 1010.40 mg/kg DM, and 634.80 mg/kg DM were noted when chitosan was added at levels of 3.87, 4.16, and 3.52. Except for Ruminococcus albus, which was not affected by increasing chitosan doses, supplementation of this additive in the concentrate quadratically increased the relative abundance of Fibrobacter succinogenes and Archaea Supplemental 740 mg CHI/kg concentrate for grazing steers receiving concentrate at 150 grams/100 kg LW is recommended to promote minimal effect on the relative population and abundance of cellulolytics and amylomatics and to restrict Archaea growth.

Degradability, in vitro fermentation parameters, and kinetic degradation of diets with increasing levels of forage and chitosan.

Chitosan is the second most important natural biopolymer in the world, extracted from crustaceans, shrimps, and crabs and can modulate rumen fermentation. Our hypothesis is that the addition of chitosan alters the fermentation patterns of different diets for ruminants. This study aimed to evaluate the effects of different levels of chitosan and forage on in vitro dry degradation kinetics and fermentation in a gas production system. The chitosan levels (0, 1625, 3,500, or 7,500 mg/kg of dry matter [DM]) were arranged in a completely randomized block design, and for in vitro ruminal fermentation assay, we used a split splot arrangement. Into the incubator, all chitosan levels were distributed in the four jars, and the forage levels varying on 100, 65, 50, 35, and 20 on DM basis. There was an interaction effect for chitosan and forage levels (P ≤ 0.05) on IVDMD; IVOMD. IVDCP and IVDNDF. Chitosan negatively affected IVDMD in all roughage levels evaluated. The pH and ammonia concentration present effect only for roughage levels and incubation hours. The chitosan did not change (P = 0.3631) the total short-chain fatty acid concentration (overall mean = 21.19 mmol/L) and the C2:C3 ratio (overall mean = 5.85). The IVDCP showed the same decreasing quadratic behavior (P < 0.0001). The increasing chitosan addition increases (P < 0.0001) the gas production and decreases (P < 0.0001) the lag time (parameter C) of diets with greater concentrate participation, characterizing greater efficiency in the degradability of the diet, confirming its potential use in diets for ruminants. Chitosan changes in vitro dry degradation kinetics and fermentation at the minimum dose of 1,722 mg/kg DM for all diets. The roughage level influenced the in vitro nutrients degradability and cumulative gas production.

Digestion, ruminal metabolism, and feeding behavior of buffaloes fed diets supplemented with soybean oil, whole and raw soybean, and calcium salts of fatty acids.

This study aimed to evaluate the effects of the inclusion of unsaturated fatty acid (UFA) sources on the nutrient intake, apparent digestibility, ruminal fermentation, and feeding behavior in diets for buffaloes. Four castrated Murrah buffaloes with approximately 24 months of age and an initial average body weight of 351 ± 15 kg were randomly assigned to a 4 × 4 Latin square experiment, containing the following diets: (1) control (CON): control diet based on soybean meal and ground corn, (2) soybean oil (SO): dietary inclusion of 2.20% (DM basis), (3) whole raw soybean (WRS): dietary inclusion of 16.0%, and (4) calcium salts of FA (CSFA): dietary inclusion of 2.60%. There was an effect of diets in ether extract intake among buffaloes fed UFA and CON diets, and among buffaloes fed CSFA and WRS diets (P < 0.05). Diets containing UFA sources provided higher EE digestibility (P < 0.05). Buffaloes fed WRS had higher rumen pH values than animals fed the CSFA diet (P < 0.05). Supplementation of UFA sources decreased the molar concentrations of short-chain fatty acids (P < 0.05). Diets influenced the times spent in chewing, idling, and the rumination efficiencies of DM and NDF (P < 0.05). The supplementation with WRS, SO, and CSFA does not negatively affect intake, digestion, ruminal metabolism, and feeding behavior. The WRS as a fat supplement source decreases dietary costs by replacing ground corn and soybean meal simultaneously compared to other fat sources used. Nevertheless, whole and raw soybean in buffaloes' diet can reduce chewing and rumination activity.

Potential degradation and colonization time of ruminal microorganisms on the particles of different oilseeds-crushed, and its chemical composition

This study was carried out to evaluate by-product of the biodiesel industry as canola, safflower, forage turnip, and soybean crushed on the chemical composition, in situ degradability, and colonization time. Canola (Brassica napus L. var. oleifera), safflower (Carthamus tinctorius L.), forage turnip (Raphanus stivus L. var. oleiferus Metzg), and soybean (Glycine max) grains went through the oil extraction process by means of a cold pressing, resulting in the oilseeds-crushed. The treatments identification included: CAN ­ Canola crushed; SAF ­ Safflower crushed; TUR ­ Forage turnip crushed; and SOY ­ Soybean crushed. The oilseed-crushed treatments were quantified about mineral (calcium, magnesium, copper, iron, manganese, zinc, phosphorus and potassium), chemical composition (dry matter, ash, organic matter, crude protein, ether extract, neutral detergent fiber, acid detergent fiber, total carbohydrates, non-fibrous carbohydrates, and total digestible nutrient contents), In situ degradability, and colonization time. Magnesium, phosphorus, and zinc showed the greater values for TUR treatment 3.46, 27.4, 39.8, respectively, when compared to the other oilseed-crushed treatments. The TUR treatment had the lowest organic matter, whereas had the greater (p≤0.05; TUR and SOY treatments) for the non-fibrous carbohydrates. Ether extract was not affected (p>0.05) with the different oilseed-crushed treatments. Neutral detergent fiber was affected (p≤0.05) for CAN and SAF treatments with the greater values, 344 and 500 g/kg of dry matter, respectively. Soluble and potentially degradable fractions for SAF treatment showed similar results. Whereas the constant rate of degradation, presented the lowest value when compared to the other treatments. Effective degradability of crude protein was greater for CAN 63.2% than SOY 65.9% treatment, which had the lowest value. Potential degradability of crude protein did not differ between treatments. Colonization time for dry matter and crude protein were similar between TUR and SOY treatments. In conclusion, oilseeds-crushed from the biodiesel production can be targeted/used, as feed with great protein and energetic potential in the ruminant's production, considering the need of correct formulation and ingredients knowledge.

Chitosan level effects on fermentation profile and chemical composition of sugarcane silage / Efeitos de níveis de quitosana sobre o perfil fermentativo e a composição química da silagem de cana-de-açúcar

This study aimed to evaluate the effects of increasing levels of chitosan (CHI) on sugarcane fermentation profile and losses, chemical composition, and in situ degradation. Treatments were: 0, 1, 2, 4, and 8 g of CHI/kg of dry matter (DM). Twenty experimental silos (PVC tubing with diameter 28 cm and height 25 cm) were used. Sand (2 kg) was placed at the bottom of each silo to evaluate effluent losses, and silos were weighed 60 d after ensiling to calculate gas losses. Samples were collected from the center of the silo mass to evaluate silage chemical composition, in situdegradation, fermentation profile, and mold and yeast count. Data were analyzed as a completely randomized design, and the treatment effect was decomposed using polynomial regression. Chitosan linearly increased acetic acid and NH3-N concentration, while yeast and mold count, and ethanol concentration decreased. Intermediary levels of CHI (from 4.47 to 6.34 g/kg DM) showed the lower values of effluent, gas, and total losses. There was a quadratic effect of CHI on the content of non-fiber carbohydrates, neutral and acid detergent, and in situ DM degradation. The lowest fiber content was observed with levels between 7.01 and 7.47 g/kg DM, whereas the highest non-fiber carbohydrate content and in situ DM degradation were found with 6.30 and 7.17 g/kg DM of CHI, respectively. Chitosan linearly increased acetic acid and NH3-N concentration, whereas it linearly reduced ethanol concentration and count of yeast and mold. Thus, intermediary levels of CHI, between 4.47 and 7.47 g/kg of DM, decrease fermentation losses and improve the nutritional value of sugarcane silage.(AU)

Foram avaliados os efeitos do aumento dos níveis de quitosana (CHI) sobre o perfil e as perdas fermentativas, a composição química e degradação in situ da silagem de cana-de-açúcar. Os tratamentos foram: 0, 1, 2, 4 e 8 g de CHI / kg de matéria seca (MS). Foram utilizados vinte silos experimentais (tubos de PVC com 28 cm de diâmetro e 25 cm de altura). Areia (2 kg) foi adicionada na porção inferior de cada silo para avaliar as perdas por efluentes e os silos foram pesados 60 dias após a ensilagem para calcular as perdas por gases. Amostras foram coletadas do centro da massa do silo para avaliar a composição química, degradação in situ, perfil fermentativo e a contagem de fungos e leveduras da silagem. Os dados foram analisados como um delineamento inteiramente casualizado e o efeito do tratamento foi decomposto usando regressão polinomial. A CHI aumentou linearmente a concentração de ácido acético e N-NH3, enquanto diminuiu a contagem de leveduras e bolores e a concentração de etanol. Os níveis intermediários de CHI (de 4,47 a 6,34 g/kg MS) mostraram os menores valores de perdas por efluentes, gases e totais. Houve efeito quadrático da CHI sobre o teor de carboidratos não fibrosos, fibra em detergente neutro e ácido e sobre a degradação in situ da MS. Os menores teores de fibras foram observados com níveis de CHI entre 7,01 e 7,47 g/kg MS, enquanto que os maiores teores de carboidratos não fibrosos e degradação in situ da MS foram encontrados com 6,30 e 7,17 g/kg MS de CHI, repectivamente. A CHI aumentou linearmente as concentrações de ácido acético e N-NH3, enquanto reduziu linearmente a concentração de etanol e a contagem de fungos e leveduras. Desta forma, níveis intermediários de CHI, entre 4,47 e 7,47 g / kg de MS, diminuem as perdas fermentativas e melhoram o valor nutricional da silagem de cana-de-açúcar.(AU)

The crambe (Crambe abyssinica Hochst) byproducts, can be used as a source of non-degradable protein in the rumen? / Coprodutos de crambe (Crambe abyssinica Hochst) podem ser utilizados como fonte de proteina não degrádavel no rúmen?

To evaluated the chemical composition and ruminal degradability of crambe byproducts (meal and crushed) and proteic supplements formulated with crushed crambe (0; 2.5; 5; 10 and 15%); five crossbred steers with average weight of 485±14 kg, were used. All the animal were kept in individual paddocks of 0.25 ha on Urochloa brizantha (syn. Brachiaria brizantha). It was observed a greater soluble fraction, higher effective degradability at 5%/h and higher degradation rate "c" and, consequent, lower indigestible fraction for crambe crushed ground in the sieve of 3 mm. The effective degradability at 5%/h was lower for the crambe crushed (55.42) in relation to the meal (48.80). The diet with 5% of inclusion of crambe showed higher effective degradability for dry matter (54.86%) and lower fraction "I" (30.64%) associated with higher fractions "c" and "b". Crambe ground in sieves of 1 and 3 mm mesh presented the highest degradability. Crushed crambe showed higher ruminal degradation than crambe meal; the crambe byproducts possibility can be use as a source of non-degradable protein in the rumen.

Para se avaliar a composição química, degradabilidade ruminal, o tempo da colonização da torta e do farelo de crambe e de suplementos protéico compostos de torta de crambe (0; 2,5; 5,0; 10 e 15%); foram utilizados cinco novilhos mestiços, com peso médio de 485 ± 14 kg. Todos os animais foram mantidos em piquetes individuais de 0,25 ha em pastagens de Urochloa brizantha (syn. Brachiaria brizantha). Observou-se uma fração solúvel maior, maior degradabilidade efetiva para a taxa de passagem de 5% / h e maior taxa de degradação "c" e, consequentemente, menor fração indigerível para a torta de crambe moído na peneira de 3 mm. A degradabilidade efetiva a 5% / h foi menor para a torta de crambe (55,42) em relação ao farelo (48,80). A dieta com adição de 5% de torta de crambe apresentou maior degradabilidade efetiva da matéria seca (54,86%) e menor fração "I" (30,64%), associada com as frações mais elevadas "c" e "b". A torta de Crambe moída em peneiras de 1 e 3 mm de diâmetro apresentaram os maiores valores de degradabilidade. A torta de apresenta maior degradação ruminal que o farelo de crambe. Os coprodutos de crambe possivelmente podem ser usados como fonte de proteína não degradada no rúmen.

Parâmetros sanguíneos de vacas leiteiras suplementadas com diferentes fontes de gordura / Blood parameters of dairy cows supplemented with different fat sources

O objetivo deste trabalho foi avaliar a utilização de diferentes fontes de gordura nas rações de vacas em lactação e seus efeitos sobre o consumo de matéria seca e as concentrações dos parâmetros sangüíneos. Foram utilizadas 12 vacas da raça Holandês, agrupadas em três quadrados latinos balanceados 4x4 e alimentadas com as seguintes rações: 1) controle; 2) óleo de soja refinado; 3) grão de soja in natura; e 4) sais de cálcio de ácidos graxos (Megalac-E). As concentrações de colesterol total, colesterol, lipoproteína de baixa densidade (LDL) e colesterol de alta densidade (HDL) foram maiores (P<0,05) para as vacas alimentadas com as rações contendo fontes de gordura em relação à ração controle. As concentrações de uréia e nitrogênio ureico no soro foram semelhantes entre as rações utilizadas, com exceção da ração contendo sais de cálcio de ácidos graxos, que apresentou concentrações menores (P<0,05). O consumo de matéria seca foi menor (P<0,05) nas vacas que receberam sais de cálcio nas rações. Os animais que receberam a ração contendo grão de soja como fonte de gordura apresentaram menor (P<0,05) produção de leite quando comparada às demais rações utilizadas. A utilização de fontes de gordura em rações de vacas em lactação alterou os parâmetros sanguíneos, dependendo da fonte de gordura utilizada.

This study was carried out to evaluate the use of different fat sources in dairy cows rations and its effects on dry matter intake, milk yield and composition, and blood parameter. Twelve Holstein cows were allocated in three balanced latin square 4x4, and fed with the following rations: 1) Control, with 2.5 percent; 2) Refined soybean oil; 3) Whole soybean; and 4), Calcium salts of fatty acids (Megalac-E). The concentrations of total cholesterol and lipoprotein low density (LDL) were higher (P<0.05) for cows that received rations with fat sources, which also causes increased (P<0.05) e concentration of high density lipoprotein (HDL). The concentrations of urea and blood urea nitrogen were similar among the rations, except for the diet containing calcium salts of fatty acids, which showed lower (P<0.05). The dry matter intake was lower (P<0.05) in cows that received calcium salts in the rations. The animals that received a diet containing whole soybeans as a source of fat had lower (P<0.05) yield milk when compared to other rations used. The use of fat sources in dairy cows rations influenced the blood parameters, especially for parameters related to lipidogram.

Avaliação de fungos predadores de nematóides do gênero Monacrosporium sobre larvas infectantes de Haemonchus contortus d caprinos / Evaluation of nematode predacios fungi of the genus Monacrosporium on infective Haemonchus contortus larvae of goats

Fungos nematófagos têm sido considerados como uma alternativa promissora para o controle biológico de nematóides gastrintestinais de ruminantes, dentre eles o gênero Monacrosporium. Dois isolados de fungos predadores de nema:, sinense SF 470 e M. appendiculatum CGI foram testados quanto a capacidade de passagem através do trato gastrinte caprinos sem perda da capacidade predatória sobre larvas infectantes(L3) de Haemonchus contortus de caprinos. Três caprinos da raça Saneen, de quatro meses de idade e do sexo masculino, foram inoculados com 5000 L3 de H. contortus. Cem gramas de pellets contendo os isolados fúngicos M. sinense SF 470 e M. appendiculatum CGI foram administrados a dois animais, por via oral, em separado. O controle foi constituído por um animal que recebeu 100 gramas de pellets sem a presença de isolados fúngicos. Amostras fecais foram coletadas às 12, 18, 24, 48, 72 e 96 horas após os tratamentos, e foram alocadas em placas de Petrie coproculturas e incubadas a 252C, por 15 dias. Houve redução significativa (p<0,05) no número de larvas recuperadas após os tratamentos dos animais com os isolados fúngicos em relação ao animal controle aproximadamente de 60%. De acordo com os resultados, pode-se afirmar a viabilidade dos fungos M. sinense e M.appendiculatum na passagem pelo trato gastrintestinal de caprinos sem perda de capacidade em predar L3 de H.Contortus de caprinos